Identification of Frankia Strains by Two-Dimensional Polyacrylamide Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Identification by Two-Dimensional Gel Electrophoresis
We have previously identified proteins in fractions of culture filtrate ofMycobacterium tuberculosis with the capacity to induce cytokine production in monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series of experiments, we have extended this technique to two-dimensional gel electrophoresis and have identified a novel 58-kDa protein ofM. tub...
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We present an approach toward standardizing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) data in support of developing a globally relevant proteomics consensus in order to provide more efficient database querying and data comparisons through the establishment of the necessary definitions and interdisciplinary reference fields for both the 2-D PAGE community, particularly in the...
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The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms. While advances in mass spectrometry have been critical, developments made in two-dimensional PAGE (2D-PAGE) have also played a major role in enabling proteomic...
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Twodimensional electrophoresis, abbreviated as 2-DE, is one of the most powerful and common tools for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens. It is an orthogonal technique that allows separation of thousands of proteins in one gel and in a two tandem electrophoretic steps where a major proportion of proteins can be r...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1984
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.47.3.489-494.1984